黄芪多糖口服吸收促进剂的研究(4)
Study on oral absorption enhancers of Astragalus polysaccharides
CHEN Xiao-yun1，2， TAN Xiao-bin1， SUN E1， LIU Dan1， JIA Xiao-bin 1，2 *， ZHANG Zhen-hai1*
(1.Key Laboratory of New Drug Delivery System of Chinese Materia Medica， Jiangsu Provincial Academy of
Chinese Medicine， Nanjing 210028， China；
2. Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract] Astragalus polysaccharides was lounded to 4-(2-aminoethylphenol)， followed by labeling the APS-Tyr with fluorescein-5-isothiocyanate (FITC) at the secondary amino group.The absorption enhancement effects of low molecular weight chitosan and protamine on astragalus polysaccharides were evaluated via Caco-2 cell culture model. The results show that the fluorecent labeling compound has good stability and high sensitivity. On the other hand low molecular weight chitosan and protamine also can promoted absorption of the astragalus polysaccharides without any cytotoxity，and the absorption increase was more significant with increasing the amount of low molecular weight chitosan and protamine. At the same time，the low molecular weight chitosan has slightly better effect. The transepithelial electric resistance (TEER) of Caco-2 cells show that absorption enhancers could improve its membrane transport permeability by opening tight junctions between cells and increasing the cell membrane fluidity.
[Key words] Astragalus polysaccharides； FITC； Caco-2 cell model； absorption enhancers
doi：10.4268/cjcmm20140719, 百拇医药(陈小云 谭晓斌 孙娥 刘丹 贾晓斌 张振海)
CHEN Xiao-yun1，2， TAN Xiao-bin1， SUN E1， LIU Dan1， JIA Xiao-bin 1，2 *， ZHANG Zhen-hai1*
(1.Key Laboratory of New Drug Delivery System of Chinese Materia Medica， Jiangsu Provincial Academy of
Chinese Medicine， Nanjing 210028， China；
2. Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract] Astragalus polysaccharides was lounded to 4-(2-aminoethylphenol)， followed by labeling the APS-Tyr with fluorescein-5-isothiocyanate (FITC) at the secondary amino group.The absorption enhancement effects of low molecular weight chitosan and protamine on astragalus polysaccharides were evaluated via Caco-2 cell culture model. The results show that the fluorecent labeling compound has good stability and high sensitivity. On the other hand low molecular weight chitosan and protamine also can promoted absorption of the astragalus polysaccharides without any cytotoxity，and the absorption increase was more significant with increasing the amount of low molecular weight chitosan and protamine. At the same time，the low molecular weight chitosan has slightly better effect. The transepithelial electric resistance (TEER) of Caco-2 cells show that absorption enhancers could improve its membrane transport permeability by opening tight junctions between cells and increasing the cell membrane fluidity.
[Key words] Astragalus polysaccharides； FITC； Caco-2 cell model； absorption enhancers
doi：10.4268/cjcmm20140719, 百拇医药(陈小云 谭晓斌 孙娥 刘丹 贾晓斌 张振海)